Genotypic and Phenotypic Applications for the Differentiation and Species-Level Identification of Achromobacter for Clinical Diagnoses
نویسندگان
چکیده
The Achromobacter is a genus in the family Alcaligenaceae, comprising fifteen species isolated from different sources, including clinical samples. The ability to detect and correctly identify Achromobacter species, particularly A. xylosoxidans, and differentiate them from other phenotypically similar and genotypically related Gram-negative, aerobic, non-fermenting species is important for patients with cystic fibrosis (CF), as well as for nosocomial and other opportunistic infections. Traditional phenotypic profile-based analyses have been demonstrated to be inadequate for reliable identifications of isolates of Achromobacter species and genotypic-based assays, relying upon comparative 16S rRNA gene sequence analyses are not able to insure definitive identifications of Achromobacter species, due to the inherently conserved nature of the gene. The uses of alternative methodologies to enable high-resolution differentiation between the species in the genus are needed. A comparative multi-locus sequence analysis (MLSA) of four selected 'house-keeping' genes (atpD, gyrB, recA, and rpoB) assessed the individual gene sequences for their potential in developing a reliable, rapid and cost-effective diagnostic protocol for Achromobacter species identifications. The analysis of the type strains of the species of the genus and 46 strains of Achromobacter species showed congruence between the cluster analyses derived from the individual genes. The MLSA gene sequences exhibited different levels of resolution in delineating the validly published Achromobacter species and elucidated strains that represent new genotypes and probable new species of the genus. Our results also suggested that the recently described A. spritinus is a later heterotypic synonym of A. marplatensis. Strains were analyzed, using whole-cell Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS), as an alternative phenotypic profile-based method with the potential to support the identifications determined by the genotypic DNA sequence-based MLSA. The MALDI-TOF MS data showed good accordance in strain groupings and identifications by the MLSA data.
منابع مشابه
Phenotypic and genotypic characterization of Candida species isolated from candideamia in Iran
Background and Purpose: Candidemia is one of the most important fungal infections caused by Candida species. Infections and mortality caused by Candida species have been on a growing trend during the past two decades. The resistance of yeasts to antifungal drugs and their epidemiological issues have highlighted the importance of accurately distinguishing the yeasts at the species level. The tec...
متن کاملDetection of Nocardia Asteroides Complex in Clinical Isolates by Real-Time Polymerase Chain Reaction
Background and Aims: Nocardia asteroides complex is the most common cause of infectious diseases due to nocardiosis. Interspecies differentiation of Nocardia genera is essential for prognosis and timely proper treatment, as well as for epidemiological studies. Since each genus has its own antibiotic resistance, precise careful diagnosis is of prime importance. As compared to biochemical and phe...
متن کاملComparative of phenotypic tests in aerobic actinomycetes
Dear editor,I read with interest article that published entitled *Isolation and identification of bioactive compound producing Rhodococcus spp. isolated from soil samples {IJMCM/5(1) (2015) 463-468}* [1]. Some of the genus such as Nocardia, Gordonia, Mycobacterium and Rhodococcus are in actinomycete family and they are Gram-positive and partially acid-fast. Rhodococcus species usually stain Gra...
متن کاملPhenotypic and Genotypic Characterization of Bifidobacterium Isolates from Healthy Adult Koreans
A total of twenty-two strict anaerobic and Gram-positive Bifidobacteria, identified as B. adolescentis, B.pseudocatenulatum, or B. longum, were isolated from healthy adult Koreans. We here investigated the cellmorphology, antimicrobial resistance patterns to novel antibiotics and genotypic differentiation ofBifidobacteria assessing repetitive DNA element PCR (rep-PCR) fingerpr...
متن کاملشناسایی و تعیین فراوانی گونه های کاندیدا جدا شده از بیماران به روش کروم آگار کاندیدا
Introduction & Objective: Candida species can cause opportunistic infections in human. Although Candida albicans is major pathogenic species, non-albicans species of Candida such as C. glabrata , C. tropicalis and C. krusei have been increasingly reported. Identification of Candida species is essential for effective antifungal therapy and for infection control purposes. In the present study the...
متن کامل